While the extensive use of large-scale genomic sequencing has greatly simplified the task of identifying peptides and proteins by mass spectrometry, the use of de novo sequencing is still required in proteomics research.

Based On Ultimate Performance
The LTQ Orbitrap XL Provides:


• Superior mass accuracy and high resolution
  leading to confident de novo sequencing results
• Multiple collision modes (CID and HCD)
  for comprehensive structural information

Spectrum of precursor ion with excellent mass accuracy.

Experimental Method


• Enzymatic enolase digest
• Online nano LC-MS and MS/MS
• Orbitrap mass analyzer full scan at R = 60,000
• Data Dependent Top3 Orbitrap MS/MS using HCD
• Dynamic exclusion

PEAKS™ view MS/MS of 1086.5445 (YGASAGNVGDEGGVAPNIQTAEEALDLIVDAIK)