Chromatographers have always been concerned with sample carryover. For the purpose of this application note, sample carryover is defined as sample from a previous injection detected during the analysis of the subsequent injections. Sample carryover is observed when the needle, injection port, transfer tube, or valve is inadequately flushed. The results show that using an adequate amount of flush volume in the Surveyor Autosampler decreases the sample carryover in classical HPLC to a negligible amount.

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